Difference between revisions of "Part:BBa K1741013"
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<partinfo>BBa_K1741013 short</partinfo> | <partinfo>BBa_K1741013 short</partinfo> | ||
− | A | + | A hairpin introduced to 5’UTR of proC slightly lowers transcription/translation. We are planning to extend it to use it as a thermometer, as for now we do not see temperature dependence. |
https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg | https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg | ||
− | This is the potential hairpin | + | This is the potential hairpin structure. |
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https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png | https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png | ||
− | The sfGFP fluorescence [RFU] was measured using Tecan | + | The sfGFP fluorescence [RFU] was measured using Tecan fluorometer. |
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+ | We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures. | ||
Latest revision as of 08:13, 27 September 2015
sfGFP under proC promoter with a hairpin in 5'UTR
A hairpin introduced to 5’UTR of proC slightly lowers transcription/translation. We are planning to extend it to use it as a thermometer, as for now we do not see temperature dependence.
This is the potential hairpin structure.
Results
Legend:
proC [BBa_K1741012]
proC with a hairpin in 5'UTR [BBa_K1741013]
proD [BBa_K1741014]
The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 232