Difference between revisions of "Part:BBa K1741012"

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<partinfo>BBa_K1741012 short</partinfo>
 
<partinfo>BBa_K1741012 short</partinfo>
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===Design===
 
  
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proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future. Expression is higher than of inducible promoters.
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proC is a moderately strong constitutive promoter (see Davis, 2010) that seems to reflect growth rate of ''E. coli'' bacterial strains on different media and potentially can be a measure of protein biosynthesis rate; to be determined in the future. Its expression rate is higher than that of inducible promoters.
  
 
===Results===
 
===Results===
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https://static.igem.org/mediawiki/parts/8/86/Teamuampoznanprochairpindiffsuagrsgraph.png
 
https://static.igem.org/mediawiki/parts/8/86/Teamuampoznanprochairpindiffsuagrsgraph.png
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https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg
 
  
 
https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png
 
https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png
  
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The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter. We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.
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The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
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We observed that proD is up to three times stronger than proC. An improvement upon this promoter would be enhancing the hairpin in proC with 5'UTR hairpin to stop translation in lower temperatures.
  
 
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Latest revision as of 08:24, 27 September 2015

sfGFP under proC promoter

proC is a moderately strong constitutive promoter (see Davis, 2010) that seems to reflect growth rate of E. coli bacterial strains on different media and potentially can be a measure of protein biosynthesis rate; to be determined in the future. Its expression rate is higher than that of inducible promoters.

Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]


Teamuamprocdiffsugarsgraph.png

Teamuampoznanprochairpindiffsuagrsgraph.png

Teamuampoznanproddiffsugarsgraph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.

We observed that proD is up to three times stronger than proC. An improvement upon this promoter would be enhancing the hairpin in proC with 5'UTR hairpin to stop translation in lower temperatures.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 201