Difference between revisions of "Part:BBa K1741003"

(→‎Design)
 
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===Design===
 
===Design===
  
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Chromosomal copy of MelR transcription factor is sufficient to activate the melibiose MelAB promoter copied from E. coli K12 chromosome (upon addition of melibiose to M9 minimal medium or to a rich medium 2xLB [0,4%]), as well as shortened MelWT with removed PstI site. In both media induction is much weaker than that of arabinose or rhamnose induced promoters.  
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MelR transcription factor translated from becterial chromosome is sufficient to activate the melibiose MelAB promoter copied from ''E. coli'' K12 chromosome (upon addition of melibiose to M9 minimal medium or to a rich medium 2xLB [0,4%]). Shortened MelWT with removed PstI site is also induced. In both media induction is much weaker than that of arabinose or rhamnose induced promoters.
  
 
Legend:
 
Legend:
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The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.  
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The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.  
  
 
We also checked the tightness of our promoters.
 
We also checked the tightness of our promoters.

Latest revision as of 09:00, 27 September 2015

sfGFP under melibiose promoter

Design

MelR transcription factor translated from becterial chromosome is sufficient to activate the melibiose MelAB promoter copied from E. coli K12 chromosome (upon addition of melibiose to M9 minimal medium or to a rich medium 2xLB [0,4%]). Shortened MelWT with removed PstI site is also induced. In both media induction is much weaker than that of arabinose or rhamnose induced promoters.

Legend:

MelS [BBa_K1741004]


UAMpoznanmelS.jpg


MelWT [BBa_K1741003]


UAMpoznanmelWT.jpg

Results

Legend:


MelS [BBa_K1741004]


MelWT [BBa_K1741003]


Teamuampoznanmelibiosem9graph.png

Teamuampoznanmelibioselbgraph.png


The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.

We also checked the tightness of our promoters.

All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose. Teamuampoznanpromoterscomparisongrpah.png




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 206