Difference between revisions of "Part:BBa K1741004"
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| − | The sfGFP fluorescence was measured using Tecan fluorometer. | + | The sfGFP fluorescence [RFU] was measured using Tecan fluorometer. |
We also checked the tightness of our promoters. | We also checked the tightness of our promoters. | ||
Latest revision as of 07:31, 27 September 2015
sfGFP under melibiose promoter (improved 5'UTR)
Twice as high expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promoter is still weaker than comercial araBAD or rhaBAD promoters, so can be the promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein.
Design
Legend:
MelS [BBa_K1741004]
MelWT [BBa_K1741003]
Results
Legend:
MelS [BBa_K1741004]
MelWT [BBa_K1741003]
The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
We also checked the tightness of our promoters.
All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 207