Difference between revisions of "Part:BBa K1741012"
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<partinfo>BBa_K1741012 short</partinfo> | <partinfo>BBa_K1741012 short</partinfo> | ||
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− | proC moderately strong constitutive promoter ( | + | proC is a moderately strong constitutive promoter (see Davis, 2010) that seems to reflect growth rate of ''E. coli'' bacterial strains on different media and potentially can be a measure of protein biosynthesis rate; to be determined in the future. Its expression rate is higher than that of inducible promoters. |
− | + | ===Results=== | |
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− | === | + | |
Legend: | Legend: | ||
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https://static.igem.org/mediawiki/parts/8/86/Teamuampoznanprochairpindiffsuagrsgraph.png | https://static.igem.org/mediawiki/parts/8/86/Teamuampoznanprochairpindiffsuagrsgraph.png | ||
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https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png | https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png | ||
− | The sfGFP fluorescence [RFU] was measured using Tecan | + | The sfGFP fluorescence [RFU] was measured using Tecan fluorometer. |
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+ | We observed that proD is up to three times stronger than proC. An improvement upon this promoter would be enhancing the hairpin in proC with 5'UTR hairpin to stop translation in lower temperatures. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 08:24, 27 September 2015
sfGFP under proC promoter
proC is a moderately strong constitutive promoter (see Davis, 2010) that seems to reflect growth rate of E. coli bacterial strains on different media and potentially can be a measure of protein biosynthesis rate; to be determined in the future. Its expression rate is higher than that of inducible promoters.
Results
Legend:
proC [BBa_K1741012]
proC with a hairpin in 5'UTR [BBa_K1741013]
proD [BBa_K1741014]
The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
We observed that proD is up to three times stronger than proC. An improvement upon this promoter would be enhancing the hairpin in proC with 5'UTR hairpin to stop translation in lower temperatures.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 201