Difference between revisions of "Part:BBa K1741000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We fused the araBAD promoter without AraC reading frame, as other sugar induced promoters to sfGFP. | + | We fused the araBAD promoter without AraC reading frame, as other sugar induced promoters to sfGFP. |
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===Source=== | ===Source=== | ||
Latest revision as of 23:18, 26 September 2015
sfGFP under arabinose promoter s1 without AraC (Arashort1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 236
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 53
Illegal SapI.rc site found at 352
Design Notes
We fused the araBAD promoter without AraC reading frame, as other sugar induced promoters to sfGFP.
Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.
References
New shorter versions of arabinose induced promoters, all originating from E. coli genome were compared to the biobrick BBa_K1481002 (https://parts.igem.org/Part:BBa_K1481002), provided last year by Poznan_Bioinf team.