Difference between revisions of "Part:BBa K1680013:Design"
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<partinfo>BBa_K1680013 short</partinfo> | <partinfo>BBa_K1680013 short</partinfo> | ||
− | + | Contains a NLS (Nuclear localisation sequence) followed by a spacer and a modified TEV-cleavage site. After Protease cleavage Phenylalanine is exposed N-terminally, which leads to rapid protein depletion. In addition to that, after cleavage a NES (Nuclear export sequence) is exposed. | |
<partinfo>BBa_K1680013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1680013 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | Shipped in | + | Shipped in RFC25 |
Latest revision as of 00:24, 26 September 2015
NDegron
Contains a NLS (Nuclear localisation sequence) followed by a spacer and a modified TEV-cleavage site. After Protease cleavage Phenylalanine is exposed N-terminally, which leads to rapid protein depletion. In addition to that, after cleavage a NES (Nuclear export sequence) is exposed.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 157 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Shipped in RFC25
Source
gBlock designed by Team Tuebingen
References
Taxis, Christof, et al. "Efficient protein depletion by genetically controlled deprotection of a dormant N‐degron." Molecular systems biology 5.1 (2009): 267.