Difference between revisions of "Part:BBa K563004:Experience"
 
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<partinfo>BBa_K563004 AddReview 4</partinfo>
 
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<I>Chalmers 2015</I>
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<I>Team Chalmers Gothenburg 2015</I>
 
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Summary: The high expression pTEF1 promoter connected to a truncated pSUC2 promoter. Improves the function of pTEF1 by adding repression at high levels of ATP and induction at low levels of ATP. For more info [https://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]
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Author: John Hellgren
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Summary: The high expression pTEF1 promoter connected to pSUC2 promoter. Can be used for induced high expression of any coding part in Saccharomyces cerevisiae at low ATP levels. Improves the function of pTEF1 by adding repression at high levels of ATP and induction at low levels of ATP. [https://parts.igem.org/Part:BBa_K1603001 pTEF1-pSUC2]
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Latest revision as of 13:54, 26 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K563004

User Reviews

UNIQf65ab78bdbf8b163-partinfo-00000000-QINU UNIQf65ab78bdbf8b163-partinfo-00000001-QINU

UNIQf65ab78bdbf8b163-partinfo-00000002-QINU

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UCSF-UCB iGEM 2014

We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter. We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter.

140630 pTEF1 GFP ROUND 1.png

The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275.

pTEF1 is also listed as parts BBa_K431008 and BBa_K319003.


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Team Chalmers Gothenburg 2015

Summary: The high expression pTEF1 promoter connected to a truncated pSUC2 promoter. Improves the function of pTEF1 by adding repression at high levels of ATP and induction at low levels of ATP. For more info BBa_K1603001