Difference between revisions of "Part:BBa K1692027"
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<h2> Overview </h2> | <h2> Overview </h2> | ||
− | + | We used the cellulose binding domain cellulosomal-scaffolding protein A (cipA) of <i> Clostridium thermocellum </i> for construction of our cipA-mSA construct ([https://parts.igem.org/Part:BBa_K1692035 BBa_K1692035]) and our spore coat construct ([https://parts.igem.org/Part:BBa_K1692028 BBa_K1692028]). The cipA used is modified to get rid of the illegal ecoRI site found in the 2014 Imperial College IGEM team construct. | |
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Latest revision as of 09:48, 24 September 2015
CIPA-MSA
Overview
We used the cellulose binding domain cellulosomal-scaffolding protein A (cipA) of Clostridium thermocellum for construction of our cipA-mSA construct (BBa_K1692035) and our spore coat construct (BBa_K1692028). The cipA used is modified to get rid of the illegal ecoRI site found in the 2014 Imperial College IGEM team construct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]