Difference between revisions of "Part:BBa K1618017"

Latest revision as of 02:14, 26 September 2015

Plac-BCD

K1618017 contains the coding sequence for the enzyme Butyryl-CoA dehydrogenase (BCD) of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Butyryl-CoA dehydrogenase catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA. At the last minute we realised that there is an EcoRI site on the gene sequence. We are working to correct this as soon as possible.

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 278
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 278
Illegal NotI site found at 960
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 278
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 278
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 278
Illegal AgeI site found at 473
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1618017 short</partinfo>
-K1618014 contains the coding sequence for the enzyme Butyryl-CoA dehydrogenase (BCD) of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Thiolase catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA
+K1618017 contains the coding sequence for the enzyme Butyryl-CoA dehydrogenase (BCD) of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Butyryl-CoA dehydrogenase catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA. At the last minute we realised that there is an EcoRI site on the gene sequence. We are working to correct this as soon as possible.
 [[File:NRP-UEA-Norwich-Flavs-Thing.png]]