Difference between revisions of "Part:BBa K1618014"

Latest revision as of 02:27, 26 September 2015

Plac-THL

K1618014 contains the coding sequence for the enzyme thiolase of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Thiolase catalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA ¹.

1. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene , Environmental Microbiology, 12(2) p. 304–14.

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1145
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1393
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1316
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1618014 short</partinfo>
-K1618014 contains the coding sequence for the enzyme butyrate kinase of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Butyrate kinase catalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA
+K1618014 contains the coding sequence for the enzyme thiolase of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Thiolase catalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA <sup>1</sup>.
+. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, <i>Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene</i> , Environmental Microbiology, 12(2) p. 304–14.
+[[File:NRP-UEA-Norwich-Flavs-Thing.png]]
+<i>Figure 1: The butyrate biosynthetic pathway. </i>
-[[File:https://static.igem.org/mediawiki/parts/9/95/NRP-UEA-Norwich-Flavs-Thing.png]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===