Difference between revisions of "Part:BBa K1639001"
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This part required for producing reporter flourescence protein GFP from Toehold-GFP(BBa_K1639000) composite part. You can find more details on [https://parts.igem.org/Part:BBa_K1639000 BBa_K1639000] | This part required for producing reporter flourescence protein GFP from Toehold-GFP(BBa_K1639000) composite part. You can find more details on [https://parts.igem.org/Part:BBa_K1639000 BBa_K1639000] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | Team [http://2017.igem.org/Team:USP-Brazil USP-Brazil 2017] has improved the characterization of this part by expressing the trigger with different concentrations of IPTG and measuring the effect of this induction on the GFP switch. | ||
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| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/8/89/T--USP-Brazil--Detection--RNA1.png"> | ||
| + | <figcaption> Induction of trigger RNA in BL21 <i>E. coli</i> cells with different concentrations of IPTG </figcaption> | ||
| + | </figure> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 16:05, 1 November 2017
Trigger RNA
This part required for producing reporter flourescence protein GFP from Toehold-GFP(BBa_K1639000) composite part. You can find more details on BBa_K1639000
Usage and Biology
Team [http://2017.igem.org/Team:USP-Brazil USP-Brazil 2017] has improved the characterization of this part by expressing the trigger with different concentrations of IPTG and measuring the effect of this induction on the GFP switch.
