Difference between revisions of "Part:BBa K1859020:Experience"
 
Line 4: Line 4:
  
 
===Applications of BBa_K1859020===
 
===Applications of BBa_K1859020===
−
 
−
===User Reviews===
 
−
<!-- DON'T DELETE --><partinfo>BBa_K1859020 StartReviews</partinfo>
 
−
<!-- Template for a user review
 
−
{|width='80%' style='border:1px solid gray'
 
−
|-
 
−
|width='10%'|
 
−
<partinfo>BBa_K1859020 AddReview number</partinfo>
 
−
<I>Username</I>
 
−
|width='60%' valign='top'|
 
−
Enter the review inofrmation here.
 
−
|};
 
−
<!-- End of the user review template -->
 
−
<!-- DON'T DELETE --><partinfo>BBa_K1859020 EndReviews</partinfo>
 
  
 
<html>
 
<html>
Line 39: Line 25:
  
 
</html>
 
</html>
 +
 +
 +
===User Reviews===
 +
<!-- DON'T DELETE --><partinfo>BBa_K1859020 StartReviews</partinfo>
 +
<!-- Template for a user review
 +
{|width='80%' style='border:1px solid gray'
 +
|-
 +
|width='10%'|
 +
<partinfo>BBa_K1859020 AddReview number</partinfo>
 +
<I>Username</I>
 +
|width='60%' valign='top'|
 +
Enter the review inofrmation here.
 +
|};
 +
<!-- End of the user review template -->
 +
<!-- DON'T DELETE --><partinfo>BBa_K1859020 EndReviews</partinfo>

Latest revision as of 05:10, 25 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859020

[iGEM gifu 2015] .

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.


User Reviews

UNIQbc8f58045b4db1bd-partinfo-00000001-QINU UNIQbc8f58045b4db1bd-partinfo-00000002-QINU