Difference between revisions of "Part:BBa K1859025"
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| − | We designed this generator [ | + | We designed this generator [BBa_K1859025] to improve circular efficiency of mRNA including His-RFP sequence. |
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859006" > [BBa_K1859006] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859006" > [BBa_K1859006] </a> | ||
), His-RFP without stop codon | ), His-RFP without stop codon | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" > [BBa_K1332002] </a> |
, Lacl | , Lacl | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | ||
, RBS | , RBS | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034" >[BBa_B0034] </a> |
and DT | and DT | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015" >[BBa_B0015] </a> |
. | . | ||
</p> | </p> | ||
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| − | We found that circular efficiency of [ | + | We found that circular efficiency of [BBa_K1859025] was the second, when we compared with amount of circular mRNA made by these generators |
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [BBa_K1332011] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [BBa_K1332011] </a> | ||
, | , | ||
Latest revision as of 09:41, 22 September 2015
mRNA circularize efficiently generator (inside complementarityⅡ)
We designed this generator [BBa_K1859025] to improve circular efficiency of mRNA including His-RFP sequence.
The generator is comprised of Circular parts combined the linker( [BBa_K1859001] and [BBa_K1859006] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
Linkers designed to adjust folding of protein are in [BBa_K1859001] and [BBa_K1859006] . RNA sequences of these linkers bind complementary with each other in circular mRNA.
We found that circular efficiency of [BBa_K1859025] was the second, when we compared with amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_K1859024] , [BBa_K1859025] , [BBa_K1859026] .