Difference between revisions of "Part:BBa K1615092"

Latest revision as of 00:29, 22 September 2015

mRFP fused to CBDcenA+Linker in RFC25

mRFP (BBa_K1615089) fused to the N terminal of CBDcenA + C terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.

We tested the dissociation of this part on Whatman 54 using ISO standard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.

We tested the dissociation of this part on Whatman 54 using ISO standard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <br>
 [[File:CBD RFP folding.jpg]]
+<br>
+<br>
+We tested the dissociation of this part on Whatman 54 using ISO standard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.
+<br>
+<br>
+[[File:BBa_K16150092.jpg]]
+<br>
+<br>
+We tested the dissociation of this part on Whatman 54 using ISO standard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.
+<br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===