Difference between revisions of "Part:BBa K1614018:Design"
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===Design Notes===
 
===Design Notes===
  
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This part is a fusion of Spinach2.1 desinged by the iGEM Team DTU Danemark. By cloning the part into the RFC 110 transcription is possible using T7 RNA Polymerase. Cleavage is achieved by the HDV.
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Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see RFC 110.
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The ATP Aptamer Spinach2.1 is generated by fusion of Spinach2.1 (designed by the iGEM Team DTU Danemark 2014) and an ATP Aptamer which exchanged the second stem loop of the Spinach2.1 construct. By exchanging the loop we achieved a ligand depencency to ATP and therefore achieved just a fluorescence in prencence of DFHBI and ATP.
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===Source===
 
===Source===

Latest revision as of 14:46, 20 September 2015

ATP Aptamer Spinach 2.1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see RFC 110. The ATP Aptamer Spinach2.1 is generated by fusion of Spinach2.1 (designed by the iGEM Team DTU Danemark 2014) and an ATP Aptamer which exchanged the second stem loop of the Spinach2.1 construct. By exchanging the loop we achieved a ligand depencency to ATP and therefore achieved just a fluorescence in prencence of DFHBI and ATP.

Source

Synthetic

References