Difference between revisions of "Part:BBa K1486003:Experience"

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We developed a new part([https://parts.igem.org/Part:BBa_K1777005 BBa_K1777005]) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.
 
We developed a new part([https://parts.igem.org/Part:BBa_K1777005 BBa_K1777005]) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.
 
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The Sao Carlos-Brazil Team has developed a yeast codon-optimized version of this part with size doubled, it can be accessed at https://parts.igem.org/Part:BBa_K3273015

Latest revision as of 21:25, 20 October 2019

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1486003

User Reviews

UNIQ97cc29f79780355c-partinfo-00000000-QINU UNIQ97cc29f79780355c-partinfo-00000001-QINU UNIQ97cc29f79780355c-partinfo-00000002-QINU

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Fudan iGEM 2015

We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.

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The Sao Carlos-Brazil Team has developed a yeast codon-optimized version of this part with size doubled, it can be accessed at https://parts.igem.org/Part:BBa_K3273015