Difference between revisions of "Part:BBa I50042"

 
 
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<partinfo>BBa_I50042 short</partinfo>
 
<partinfo>BBa_I50042 short</partinfo>
  
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pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
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[[Part:BBa_I50042|BBa_I50042]] is a pSC101 origin of replication.  [[Part:BBa_I50042|BBa_I50042]] is the replication origin found in the pSB4*5 series of BioBrick vectors.
  
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===Usage and Biology===
 
===Usage and Biology===
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*[[Part:BBa_I50042|BBa_I50042]] yields vectors with an expected copy number of ~5 per cell ([http://openwetware.org/wiki/Molecular_Cloning Molecular Cloning]).
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===References===
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'''Engineering BioBrick vectors from BioBrick parts'''<br>
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''Journal of Biological Engineering,'' 2008 Apr 14;2:5<br>
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Reshma Shetty, Drew Endy, Tom Knight<br>
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[http://www.jbioleng.org/content/2/1/5 URL]<br>
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656107 GenBank EU496096]
  
 
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Latest revision as of 23:23, 25 September 2012

pSC101 origin of replication

BBa_I50042 is a pSC101 origin of replication. BBa_I50042 is the replication origin found in the pSB4*5 series of BioBrick vectors.

Usage and Biology

  • BBa_I50042 yields vectors with an expected copy number of ~5 per cell ([http://openwetware.org/wiki/Molecular_Cloning Molecular Cloning]).

References

Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656107 GenBank EU496096]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]