Difference between revisions of "Part:BBa K1648004:Experience"
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Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into <i>A. vinelandii</i> genome via <b>random integration</b>. | Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into <i>A. vinelandii</i> genome via <b>random integration</b>. | ||
| − | A GFP-nanobody([https://parts.igem.org/Part: | + | A GFP-nanobody([https://parts.igem.org/Part:BBa_K1648005 K1648005]) is added to this part in order to express GFP-nanobody on the magnetosome. This new part is [https://parts.igem.org/Part:BBa_K1648006 K1648006] |
This Part were verified by double digestion (Fig. 1). | This Part were verified by double digestion (Fig. 1). | ||
Latest revision as of 05:23, 20 September 2015
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1648004
Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into A. vinelandii genome via random integration.
A GFP-nanobody(K1648005) is added to this part in order to express GFP-nanobody on the magnetosome. This new part is K1648006
This Part were verified by double digestion (Fig. 1).
Fig 1. The photo of 1% agarose gel electrophoresis showing PCR products of operons in Magnetosome Island (MAI). L: DNA ladder. Lane 1: mamHIEJKLMN. Lane 2: mamOPQRBSTU. Lane 3: mamPQRBSTU. Lane 4: mamYXZftsZ-like. Lane 5: mamGFDC. Lane 6: mamGFD. Lane 7: mms6.
RFP is inserted in Insertion Kit for biobrick standard. The Insertion kit with RFP is transformed in E. coli (DH5 alpha). Expression of RFP in Insertion is shown(Fig 2.).
Fig 2. Insertion kit with RFP(BBa_K1648004) is expressed in E. coli (DH5 alpha)
User Reviews
UNIQ61d1f1e74b03473f-partinfo-00000000-QINU UNIQ61d1f1e74b03473f-partinfo-00000001-QINU