Difference between revisions of "Part:BBa K1676075"
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<partinfo>BBa_K1676075 short</partinfo> | <partinfo>BBa_K1676075 short</partinfo> | ||
| − | This reporter carries a mutant of RBS BBa_B0034. This mutant RBS carries a total of 7 base substitutions. This RBS mutants is mutated based on the results of our | + | This reporter carries a mutant of RBS BBa_B0034. This mutant RBS carries a total of 7 base substitutions. This RBS mutants is mutated based on the results of our BBa_B0034 mutant library. Substitutions of basepairs with the highest GFP expression is carried out. The construct consist of the pLac promoter BBa_R0011, mutant RBS derived from BBa_B0034, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain. |
Previous results of mutant library: | Previous results of mutant library: | ||
Latest revision as of 02:57, 20 September 2015
GFP Reporter with Mutant RBS Opt
This reporter carries a mutant of RBS BBa_B0034. This mutant RBS carries a total of 7 base substitutions. This RBS mutants is mutated based on the results of our BBa_B0034 mutant library. Substitutions of basepairs with the highest GFP expression is carried out. The construct consist of the pLac promoter BBa_R0011, mutant RBS derived from BBa_B0034, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.
Previous results of mutant library:
As we can see mutations on basepair 5, 6, 7, 8 and 9 brings to a lower GFP expression. Hence, no substitutions are made on these basepairs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725