Difference between revisions of "Part:BBa K1846001"
 
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<partinfo>BBa_K1846001 short</partinfo>
 
<partinfo>BBa_K1846001 short</partinfo>
  
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The tfa (tail fibre assembly) protein of bacteriophage Lambda assists in the assembly of the stf (short tail fibre) protein into a functional tail fibre. This part provides the gene sequence for tfa, together with a TetR repressible promoter (TetO, available separately as BioBrick part BBa_R0040), ribosome binding site (available separately as part BBa_B0034) and an rrNB T1 terminator (available separately as BioBrick part BBa_B0010).
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This part provides the gene sequence for tfa (tail fibre assembly) protein, together with a TetR repressible promoter (TetO, available separately as BioBrick part [https://parts.igem.org/Part:BBa_R0040 BBa_R0040]), ribosome binding site (available separately as part [https://parts.igem.org/Part:BBa_B0034 BBa_B0034]) and an rrNB T1 terminator (available separately as BioBrick part [https://parts.igem.org/Part:BBa_B0010 BBa_B0010]).
  
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Characterisation of this part, under TetR regulation, is available separately - see BioBrick BBa_K1846007; https://parts.igem.org/Part:BBa_K1846007.
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===Usage and Biology===
  
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The tfa (tail fibre assembly) protein of bacteriophage lambda assists in the assembly of the stf (short tail fibre) protein into a functional short tail fibre [1],[2]. ''Tfa'' gene displays a high level of homology (~40%) with the gp38 of bacteriophage T4 [2],[3],[4].
  
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===Usage and Biology===
 
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1846001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1846001 SequenceAndFeatures</partinfo>
  
  
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===Characterisation===
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===Functional Parameters===
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<partinfo>BBa_K1846001 parameters</partinfo>
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Characterisation of this part, under TetR regulation, is available via [https://parts.igem.org/Part:BBa_K1846007 BBa_K1846007] composite part.
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===References===
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[1] Hashemolhosseini, S., Stierhof, Y. D., Hindennach, I., & Henning, U. (1996). Characterization of the helper proteins for the assembly of tail fibers of coliphages T4 and λ. Journal of Bacteriology, 178(21), 6258–6265.
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[2] Montag, D., Schwarz, H., & Henning, U. (1989). A component of the side tail fiber of E. coli bacteriophage λ can functionally replace the receptor-recognizing part of a long tail fiber protein of the unrelated bacteriophage T4. Journal of Bacteriology, 171(8), 4378–4384.
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[3] Hendrix, R. W., & Duda, R. L. (1992). Bacteriophage lambda PaPa: not the mother of all lambda phages. Science (New York, N.Y.), 258(5085), 1145–1148. doi:10.1126/science.1439823
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[4] Haggard-Ljungquist, E., Halling, C., & Calendar, R. (1992). DNA sequences of the tail fiber genes of bacteriophage P2: Evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages. Journal of Bacteriology, 174(5), 1462–1477.

Latest revision as of 18:36, 20 September 2015

Bacteriophage lambda tail fibre assembly protein circuit

This part provides the gene sequence for tfa (tail fibre assembly) protein, together with a TetR repressible promoter (TetO, available separately as BioBrick part BBa_R0040), ribosome binding site (available separately as part BBa_B0034) and an rrNB T1 terminator (available separately as BioBrick part BBa_B0010).


Usage and Biology

The tfa (tail fibre assembly) protein of bacteriophage lambda assists in the assembly of the stf (short tail fibre) protein into a functional short tail fibre [1],[2]. Tfa gene displays a high level of homology (~40%) with the gp38 of bacteriophage T4 [2],[3],[4].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

Characterisation of this part, under TetR regulation, is available via BBa_K1846007 composite part.


References

[1] Hashemolhosseini, S., Stierhof, Y. D., Hindennach, I., & Henning, U. (1996). Characterization of the helper proteins for the assembly of tail fibers of coliphages T4 and λ. Journal of Bacteriology, 178(21), 6258–6265.

[2] Montag, D., Schwarz, H., & Henning, U. (1989). A component of the side tail fiber of E. coli bacteriophage λ can functionally replace the receptor-recognizing part of a long tail fiber protein of the unrelated bacteriophage T4. Journal of Bacteriology, 171(8), 4378–4384.

[3] Hendrix, R. W., & Duda, R. L. (1992). Bacteriophage lambda PaPa: not the mother of all lambda phages. Science (New York, N.Y.), 258(5085), 1145–1148. doi:10.1126/science.1439823

[4] Haggard-Ljungquist, E., Halling, C., & Calendar, R. (1992). DNA sequences of the tail fiber genes of bacteriophage P2: Evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages. Journal of Bacteriology, 174(5), 1462–1477.