Difference between revisions of "Part:BBa K1616025"

 
 
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<partinfo>BBa_K1616025 short</partinfo>
 
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NanoLuc (Nluc)is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter.  
 
NanoLuc (Nluc)is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter.  
 
NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
 
NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
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Thermal stable enzyme
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NanoLuc retains activity following 30 min incubation at 55 °C.
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Active over broad pH range
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NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7.
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Monomeric enzyme
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NanoLuc facilitates use as transcriptional reporter or fusion partner.
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<center>
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https://static.igem.org/mediawiki/2015/e/e6/NanoLuc.png
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</center>
  
 
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<partinfo>BBa_K1616025 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1616025 SequenceAndFeatures</partinfo>
  
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===References===
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Kyle Hooper, Ph.D, Applications of a smaller, brighter, more versatile luciferase:NanoLuc™ Luciferase Technology, 2012
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<br>Shreeram Akilesh, Bioluminescence: Nature’s Bright Idea,2000.
  
 
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Latest revision as of 17:33, 19 September 2015

NanoLuc

NanoLuc (Nluc)is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter. NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.


Thermal stable enzyme NanoLuc retains activity following 30 min incubation at 55 °C. Active over broad pH range NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7. Monomeric enzyme NanoLuc facilitates use as transcriptional reporter or fusion partner.

NanoLuc.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Kyle Hooper, Ph.D, Applications of a smaller, brighter, more versatile luciferase:NanoLuc™ Luciferase Technology, 2012
Shreeram Akilesh, Bioluminescence: Nature’s Bright Idea,2000.