Difference between revisions of "Part:BBa J69511"
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<partinfo>BBa_J69511 short</partinfo> | <partinfo>BBa_J69511 short</partinfo> | ||
| − | Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct | + | Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct, BBa_B0034 |
| − | 1AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases. | + | 1AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases. These mutants were then characterise in Shewanella oneidensis MR1. |
| − | Our library entry can be found from BBa_K1676037 to BBa_K1676072. | + | Our library entry can be found from BBa_K1676037 to BBa_K1676072 where GFP expression monitoring data can be found. :) |
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| + | https://static.igem.org/mediawiki/2015/4/49/MUTATIONS.png | ||
Results of the mutant library: | Results of the mutant library: | ||
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https://static.igem.org/mediawiki/2015/3/3a/Rbs-spots.jpeg | https://static.igem.org/mediawiki/2015/3/3a/Rbs-spots.jpeg | ||
| − | + | https://static.igem.org/mediawiki/2015/9/90/Sumary.png | |
Latest revision as of 04:58, 20 September 2015
GFP reporter device - LacI repressible
Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct, BBa_B0034
1AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases. These mutants were then characterise in Shewanella oneidensis MR1.
Our library entry can be found from BBa_K1676037 to BBa_K1676072 where GFP expression monitoring data can be found. :)
Results of the mutant library:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725