Difference between revisions of "Part:BBa K1676037"
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This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the first base pair. The first base pair A is substituted to C. | This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the first base pair. The first base pair A is substituted to C. | ||
| − | The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , | + | The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015. |
The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain. | The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain. | ||
Latest revision as of 15:02, 19 September 2015
GFP Reporter with Mutant RBS 1AC
This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the first base pair. The first base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.
The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.
This reporter can also be derived from the existing construct, BBa_J69511.
The characterization is shown below,
Growth curve:
GFP Reading:
It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725