Difference between revisions of "Part:BBa K1648005"

Latest revision as of 05:29, 20 September 2015

GFP-nanobody

The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [K929102].

Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.

L: DNA ladder. Lane 2: GFP-nanobody.

This Part is added to Insertion Kit(K1648004), forming a new Part(K1648006), which is used to express GFP-nanobody on the membrane of magnetosome.

This Part were verified by double digestion (Fig. 2) and sequencing.

Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K929102 K929102]].
+[[File:PCR GFPnanobody.jpg|350px]]
+Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.
-[[File:GFP-nanobody.jpg|350px]]
+L: DNA ladder. Lane 2: GFP-nanobody.
-Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.
-          L: DNA ladder. Lane 2: GFP-nanobody.
+This Part is added to Insertion Kit([https://parts.igem.org/Part:BBa_K1648004 K1648004]), forming a new Part([https://parts.igem.org/Part:BBa_K1648006 K1648006]), which is used to express GFP-nanobody on the membrane of magnetosome.
 This Part were verified by double digestion (Fig. 2) and sequencing.