Difference between revisions of "Part:BBa K1648005"
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The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K929102 K929102]]. | The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K929102 K929102]]. | ||
| + | [[File:PCR GFPnanobody.jpg|350px]] | ||
| + | Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products. | ||
| − | + | L: DNA ladder. Lane 2: GFP-nanobody. | |
| − | Figure | + | |
| + | This Part is added to Insertion Kit([https://parts.igem.org/Part:BBa_K1648004 K1648004]), forming a new Part([https://parts.igem.org/Part:BBa_K1648006 K1648006]), which is used to express GFP-nanobody on the membrane of magnetosome. | ||
| + | |||
| + | This Part were verified by double digestion (Fig. 2) and sequencing. | ||
| + | |||
| + | [[File:GFPnano-c.JPG|350px]] | ||
| + | |||
| + | Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. | ||
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Latest revision as of 05:29, 20 September 2015
GFP-nanobody
The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [K929102].
Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.
L: DNA ladder. Lane 2: GFP-nanobody.
This Part is added to Insertion Kit(K1648004), forming a new Part(K1648006), which is used to express GFP-nanobody on the membrane of magnetosome.
This Part were verified by double digestion (Fig. 2) and sequencing.
Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]