Difference between revisions of "Part:BBa K1734007"
 
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<partinfo>BBa_K1734007 short</partinfo>
 
<partinfo>BBa_K1734007 short</partinfo>
  
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C1 description
 
  
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===Usage and Biology===
 
===Usage and Biology===
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This construct consists of a promoter p10, the Honeybee Melittin signal peptide, and the Nanoluc coding sequence with a Histidine Tag, a T2A sequence for polycistronic expression, and and finally RFP protein with Histidine Tag.
 
This construct consists of a promoter p10, the Honeybee Melittin signal peptide, and the Nanoluc coding sequence with a Histidine Tag, a T2A sequence for polycistronic expression, and and finally RFP protein with Histidine Tag.
  
 
Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.  
 
Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.  
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1734007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1734007 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:56, 19 September 2015

C1 (p10 + HBM signal sequence + Nanoluc + T2A sequence + mRFP1 + 6xHis)


Usage and Biology

This construct consists of a promoter p10, the Honeybee Melittin signal peptide, and the Nanoluc coding sequence with a Histidine Tag, a T2A sequence for polycistronic expression, and and finally RFP protein with Histidine Tag.

Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5
    Illegal SapI site found at 641