Difference between revisions of "Part:BBa K1760001"
 
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<partinfo>BBa_K1760001 short</partinfo>
 
<partinfo>BBa_K1760001 short</partinfo>
  
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TodE expresses Catechol 2-3 dioxygenase
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Designed biobrick is a composite part that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD. In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzyme
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Designed biobrick is a coding sequence that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. TodE expresses Catechol 2-3 dioxygenase protein. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD.  
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[[File:Toronto_2015_Pathway.png]]
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In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzymes.
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[[File:TodEonly.png]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:27, 19 September 2015

TodE


Designed biobrick is a coding sequence that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. TodE expresses Catechol 2-3 dioxygenase protein. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD.


Toronto 2015 Pathway.png


In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzymes.


TodEonly.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]