Difference between revisions of "Part:BBa K1679019"

Latest revision as of 03:20, 19 September 2015

GFP+Promoter+RNA thermometer (FourU) +mRFP

This is a Translational_Unit containing, a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040), two terminator(BBa_B0010 and BBa_B0012), a promoter(BBa_J23119K),a RNA thermometer (BBa_K115002) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.

This is Translational_Unit that a GFP reporter device except promoter in front of a RFP reprorter device. It's a intermediate before we constructing BBa_K1679030

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 890
Illegal NheI site found at 913
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1539
Illegal AgeI site found at 1651
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 662

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1679019 short</partinfo>
-This is a Translational_Unit containing,a RBS (BBa_B0034),a GFP coding sequence (BBa_E0040) ,two terminator(BBa_B0010 and BBa_B0012),a promoter(BBa_J23119K),,a RNA thermometer (BBa_K115002) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.
+This is a Translational_Unit containing, a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040), two terminator(BBa_B0010 and BBa_B0012), a promoter(BBa_J23119K),a RNA thermometer (BBa_K115002) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into ''E.coli''.
 This is Translational_Unit that a GFP reporter device except promoter in front of a RFP reprorter device.
+It's a intermediate before we constructing [https://parts.igem.org/Part:BBa_K1679030 BBa_K1679030]
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1679019 SequenceAndFeatures</partinfo>