Difference between revisions of "Part:BBa K1602025"
 
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                 This part is encodes for the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield.  
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                 This part is encodes for the green fluorescent protein (<b>GFP</b>). It also contains a strong Ribosome Binding Site (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>). Also the sequence of a Histag is fused to the gene sequence, improving the purification yield. Fusing the Si4-tag(<a href:"https://parts.igem.org/Part:BBa_K1028000">BBa_K1028000</a>) to the 3' end of the sequence enables the immobilization of the multi-complex enzyme to a silica surface.
 
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Latest revision as of 02:14, 19 September 2015

HisTag-GFP_Si4-Tag fusion protein with RBS BBa_B0034

This part is encodes for the green fluorescent protein (GFP). It also contains a strong Ribosome Binding Site (BBa_B0034). Also the sequence of a Histag is fused to the gene sequence, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence enables the immobilization of the multi-complex enzyme to a silica surface.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 680