Difference between revisions of "Part:BBa K1807003:Experience"
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===Applications of BBa_K1807003=== | ===Applications of BBa_K1807003=== | ||
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| + | Figure 1. Graph showing the phosphate concentration within the cell lysate of 10^8, cells for 5 different ppk variants after 6 hours; ; Δppk Ap SK12 - Candidatus ''Accumulibacter phosphatis'' SK-12 strain ppk variant, Δppk Ap UW1- Candidatus ''Accumulibacter phosphatis'' UW-1 strain ppk variant, Δppk Ap BA91- Candidatus ''Accumulibacter phosphatis'' BA-91 strain ppk variant, Δ ppk is ''Escherichia coli'' with ppk knockout using kanamycin KEIO collection. BW25113 is the ''Escherichia coli'' strain used for the experiment. One way ANOVA was used and overall there was a statistically significant difference between groups and post-hoc LSD was used; NS signifies no statistical significance, *** signifies p<0.001. (ANOVA: F=48.503; d.f. = 14, p<0.001) Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations calculated from known phosphate concentration standards. | ||
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| + | Figure 2. Graph showing uptake of phosphate after 6 hours from the media for 5 different ppk variants standardized against cell amount (1 OD600); Δppk Ac SK12 - Candidatus ''Accumulibacter phosphatis'' SK-12 strain ppk variant, Δppk Ac UW1- Candidatus ''Accumulibacter phosphatis'' UW-1 strain ppk variant, Δppk Ac BA91- Candidatus ''Accumulibacter phosphatis'' BA91 strain ppk variant, Δ ppk is ''Escherichia coli'' with ppk knockout using kanamycin KEIO collection. BW25113 is the ''Escherichia coli'' strain used for the experiment. One way ANOVA was used and the result showed overall there was statistically significant different. Post-hoc LSD was used; NS signifies no statistical significance, * signifies p<0.05. (ANOVA: F=3.810; d.f. = 14, p<0.05) Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations calculated from known phosphate concentration standards. | ||
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| + | ===Result=== | ||
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| + | The Ap PPK BA-91 protein generator does show very significant increase in phosphate uptake/storage. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 04:00, 19 September 2015
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how you used this part and how it worked out.
Applications of BBa_K1807003
Figure 1. Graph showing the phosphate concentration within the cell lysate of 10^8, cells for 5 different ppk variants after 6 hours; ; Δppk Ap SK12 - Candidatus Accumulibacter phosphatis SK-12 strain ppk variant, Δppk Ap UW1- Candidatus Accumulibacter phosphatis UW-1 strain ppk variant, Δppk Ap BA91- Candidatus Accumulibacter phosphatis BA-91 strain ppk variant, Δ ppk is Escherichia coli with ppk knockout using kanamycin KEIO collection. BW25113 is the Escherichia coli strain used for the experiment. One way ANOVA was used and overall there was a statistically significant difference between groups and post-hoc LSD was used; NS signifies no statistical significance, *** signifies p<0.001. (ANOVA: F=48.503; d.f. = 14, p<0.001) Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations calculated from known phosphate concentration standards.
Figure 2. Graph showing uptake of phosphate after 6 hours from the media for 5 different ppk variants standardized against cell amount (1 OD600); Δppk Ac SK12 - Candidatus Accumulibacter phosphatis SK-12 strain ppk variant, Δppk Ac UW1- Candidatus Accumulibacter phosphatis UW-1 strain ppk variant, Δppk Ac BA91- Candidatus Accumulibacter phosphatis BA91 strain ppk variant, Δ ppk is Escherichia coli with ppk knockout using kanamycin KEIO collection. BW25113 is the Escherichia coli strain used for the experiment. One way ANOVA was used and the result showed overall there was statistically significant different. Post-hoc LSD was used; NS signifies no statistical significance, * signifies p<0.05. (ANOVA: F=3.810; d.f. = 14, p<0.05) Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations calculated from known phosphate concentration standards.
Result
The Ap PPK BA-91 protein generator does show very significant increase in phosphate uptake/storage.
User Reviews
UNIQb49d2396d9c64f01-partinfo-00000002-QINU UNIQb49d2396d9c64f01-partinfo-00000003-QINU