Difference between revisions of "Part:BBa K1734008"
 
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description
 
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===Usage and Biology===
 
===Usage and Biology===
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This construct consists of the polyhedrin promoter, a Honeybee Melittin signal peptide and the Nanoluc  with a Histidine Tag, the T2A sequence for polycistronic expression, and finally RFP protein with Histidine Tag.
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Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K1734008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1734008 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:56, 19 September 2015

C2 (pPH + HBM signal sequence + Nanoluc + T2A sequence + mRFP1 + 6xHis)


Usage and Biology

This construct consists of the polyhedrin promoter, a Honeybee Melittin signal peptide and the Nanoluc with a Histidine Tag, the T2A sequence for polycistronic expression, and finally RFP protein with Histidine Tag.

Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 699
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 623