Difference between revisions of "Part:BBa K1631012"
(→‎Usage and Biology)
 
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We succeeded to control growth rate of <i>E coli</i>.
 
We succeeded to control growth rate of <i>E coli</i>.
 
<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
 
<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
 +
We measured OD600 of 3 samples for each condition.
 
[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
 
[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
 
</p>
 
</p>

Latest revision as of 02:38, 19 September 2015

Plac -rbs- Barnase -rbs- GFP -d.term

Barnase and GFP is co-expressed by IPTG induction. This is composite parts of BBa_K1631000 which is improvement of BBa_I76211.

If you use this part, you can estimated the expression level of barnase and evaluate its toxic activity correspond to its expression level.

Usage and Biology

We succeeded to control growth rate of E coli.

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. We measured OD600 of 3 samples for each condition.

alt text

We also evaluated the growth rate by slope of next graph.

alt text


Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1081