Difference between revisions of "Part:BBa K1813013"

Latest revision as of 00:42, 19 September 2015

CYP2D6 Expression Cassette

A construct used to achieve the overexpression of CYP2D6 (BBa_K11813005) in a bacterial host. Structure: ptac:: HUMCYPDB1:: term.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 410
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1000
1000
COMPATIBLE WITH RFC[1000]

Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP2D6 by a bacterial organism harboring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813024 and BBa_K1813032.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1813013 short</partinfo>
-A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K11813005</b></a></html>) in a bacterial host.
+A construct used to achieve the overexpression of CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K11813005</b></a></html>) in a bacterial host.
 Structure: ptac:: HUMCYPDB1:: term.
@@ Line 21: / Line 21: @@
 <h2>Background</h2>
-Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of HUMCYPDB1 by a bacterial organism harboring this construct.
+Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP2D6 by a bacterial organism harboring this construct.
 This construct was assembled by Michael VanInsberghe, and is codon optimized for <i> E. coli </i>. <br>
 It is used in <html><b><a href="https://parts.igem.org/Part:BBa_K1813024">BBa_K1813024</b></a></html> and <html><b><a href="https://parts.igem.org/Part:BBa_K1813032">BBa_K1813032</b></a></html>.