Difference between revisions of "Part:BBa K1583001"
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<partinfo>BBa_K1583001 short</partinfo> | <partinfo>BBa_K1583001 short</partinfo> | ||
− | CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of <i>E.coli</i> (Barnhart & Chapman 2010). | + | <html> |
+ | <p>CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of <i>E.coli</i> (Barnhart & Chapman 2010).</p> | ||
− | + | <p><i>csgC</i> is used in the biobricks: | |
− | <ul><li><a href="https://parts.igem.org/Part:BBa_K1583200">BBa_K1583200</a> | + | <ul><li><a href="https://parts.igem.org/Part:BBa_K1583200"link+_blank">BBa_K1583200</a></li> |
− | <li><a href="https://parts.igem.org/Part:BBa_K1583201">BBa_K1583201</a>< | + | <li><a href="https://parts.igem.org/Part:BBa_K1583201"link+_blank">BBa_K1583201</a></li> |
− | <li><a href="https://parts.igem.org/Part:BBa_K1583202">BBa_K1583202</a>< | + | <li><a href="https://parts.igem.org/Part:BBa_K1583202"link+_blank">BBa_K1583202</a></li> |
− | <li><a href="https://parts.igem.org/Part:BBa_K1583203">BBa_K1583203</a>< | + | <li><a href="https://parts.igem.org/Part:BBa_K1583203"link+_blank">BBa_K1583203</a></li> |
− | <li><a href="https://parts.igem.org/Part:BBa_K1583204">BBa_K1583204</a>< | + | <li><a href="https://parts.igem.org/Part:BBa_K1583204"link+_blank">BBa_K1583204</a></li> |
− | <li><a href="https://parts.igem.org/Part:BBa_K1583205">BBa_K1583205</a>< | + | <li><a href="https://parts.igem.org/Part:BBa_K1583205"link+_blank">BBa_K1583205</a></li></ul></p> |
− | The first three parts contain CsgC with a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>). | + | <p>The first three parts contain CsgC with a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>), respectively.</p> |
+ | <p>The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>), respectively.</p> | ||
− | + | </html> | |
− | + | ||
<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><h3>Sequence and Features</h3></span> |
<partinfo>BBa_K1583001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1583001 SequenceAndFeatures</partinfo> | ||
<h3>References</h3> | <h3>References</h3> | ||
<p>Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.</p> | <p>Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.</p> |
Latest revision as of 00:24, 19 September 2015
CsgC
CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli (Barnhart & Chapman 2010).
csgC is used in the biobricks:
The first three parts contain CsgC with a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100), respectively.
The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100), respectively.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.