Difference between revisions of "Part:BBa K1758101"

Latest revision as of 19:20, 19 September 2015

Translation enhancing 5-UTR + sfGFP

This part includes our designed, translation enhancing 5'-untranslated region (5'-UTR; BBa_K1758100), in front of sfGFP coding sequence (BBa_I746916). It also contains a double terminator made of B0010 and B0012. The translation enhancing sequence improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in in vitro cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).

Usage and Biology

By adding a promoter of choice, sfGFP is produced. The high efficiency of our designed 5'-UTR ensures reporter production even when weak promoters are employed.

Characterization

This part has been used in a variety of experiments, including experiments with BBa_K1758102, BBa_K1758377, BBa_K1758312, BBa_K1758314, BBa_K1758323, BBa_K1758325, BBa_K1758332 and others. For details see the corresponding part or our wiki. The sequence was in general cloned via Gibson assembly.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 59

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1758101 short</partinfo>
-This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I746916 BBa_I746916]). It also contains a double terminator made of [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 B0010] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 B0012]. The translation enhancing sequence improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in ''in vitro'' cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).
+This part includes our designed, translation enhancing 5'-untranslated region <html>(5'-UTR; <a href="https://parts.igem.org/Part:BBa_K1758100">BBa_K1758100</a>)</html>, in front of sfGFP coding sequence ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I746916 BBa_I746916]). It also contains a double terminator made of [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 B0010] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 B0012]. The translation enhancing sequence improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in ''in vitro'' cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).
@@ Line 11: / Line 11: @@
 <h2> Characterization </h2>
-<p> This part has been used in a variety of experiments, including experiments with BBa_K1758102, BBa_K1758377, BBa_K1758312, BBa_K1758314, BBa_K1758323, BBa_K1758325, BBa_K1758332 and others. For details see the corresponding part or our wiki. </p>
+<p> This part has been used in a variety of experiments, including experiments with BBa_K1758102, BBa_K1758377, BBa_K1758312, BBa_K1758314, BBa_K1758323, BBa_K1758325, BBa_K1758332 and others. For details see the corresponding part or our wiki. The sequence was in general cloned via Gibson assembly. </p>