Difference between revisions of "Part:BBa K632003:Experience"

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We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light.
 
We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light.
 
We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator.
 
We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator.
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https://static.igem.org/mediawiki/2015/f/f2/Team_HZAU-China_Fig_W4-1.png
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https://static.igem.org/mediawiki/2015/a/a7/Team_HZAU-China_Fig_W4-2.png
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https://static.igem.org/mediawiki/2015/0/06/Hzau1.jpg
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https://static.igem.org/mediawiki/2015/8/8a/Hzau2.jpg
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Fig 1: The genetic circuit of test system.
 
Fig 1: The genetic circuit of test system.
 
<br>
 
<br>
 
Two sample owning the same genetic circuit were set in our test experiment, the one in dark, the other in red light, and each sample had three biologic repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture shown.
 
Two sample owning the same genetic circuit were set in our test experiment, the one in dark, the other in red light, and each sample had three biologic repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture shown.
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https://static.igem.org/mediawiki/2015/0/09/Hzau3.jpg
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<br>
 
Fig 2:A red LED powered by battery is set at the bottom of a cup to provide red light
 
Fig 2:A red LED powered by battery is set at the bottom of a cup to provide red light
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https://static.igem.org/mediawiki/2015/f/fc/Hzau4.jpg
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Fig 3:Two light conditions in our test device.
 
Fig 3:Two light conditions in our test device.
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<br>
 
<br>
 
After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes. (In the test, we used GFP as the target gene).
 
After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes. (In the test, we used GFP as the target gene).
 +
https://static.igem.org/mediawiki/2015/2/29/Hzau5.jpg
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Fig 4. The effect of red light on the expression of genes
 
Fig 4. The effect of red light on the expression of genes
 
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More information, click here: http://2015.igem.org/Team:HZAU-China
 
More information, click here: http://2015.igem.org/Team:HZAU-China
 
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Latest revision as of 00:41, 19 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K632003

User Reviews

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No review score entered. HZAU-China iGEM 2015


We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light. We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator.

Hzau1.jpg Hzau2.jpg

Fig 1: The genetic circuit of test system.
Two sample owning the same genetic circuit were set in our test experiment, the one in dark, the other in red light, and each sample had three biologic repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture shown. Hzau3.jpg
Fig 2:A red LED powered by battery is set at the bottom of a cup to provide red light

Hzau4.jpg

Fig 3:Two light conditions in our test device.


After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes. (In the test, we used GFP as the target gene). Hzau5.jpg

Fig 4. The effect of red light on the expression of genes

More information, click here: http://2015.igem.org/Team:HZAU-China

;

UNIQb24acb8efcbb3f6f-partinfo-00000002-QINU