Difference between revisions of "Part:BBa K1631012:Experience"

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===User Reviews===
 
===User Reviews===
 
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We succeeded to control growth rate of <i>E coli</i>.
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We succeeded to control growth rate of <i>E coli JM109</i> (which expresses LacI excessively).
 
<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
 
<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
 
[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
 
[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
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We also evaluated the growth rate by slope of next graph.
 
We also evaluated the growth rate by slope of next graph.
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[[File:Yamanaka 001.png|600px|thumb|alt text]]
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[[File:Yamanaka 001.png|600px|thumb|center|alt text]]
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PlacGFP-           0.0213
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<br>Plac GFP-         0.0213(/min)
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PlacGFP+           0.0205
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<br>Plac GFP+         0.0205(/min)
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Plac Barnase GFP-  0.0222
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<br>Plac Barnase GFP-  0.0222(/min)
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Plac Barnase GFP+  0.0117
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<br>Plac Barnase GFP+  0.0117(/min)
  
  

Latest revision as of 00:50, 19 September 2015

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Please enter how you used this part and how it worked out.

Applications of BBa_K1631012

User Reviews

UNIQ873723eac7b7e7b8-partinfo-00000000-QINU We succeeded to control growth rate of E coli JM109 (which expresses LacI excessively).

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.

alt text

We also evaluated the growth rate by slope of next graph.

alt text


Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)


UNIQ873723eac7b7e7b8-partinfo-00000001-QINU