Difference between revisions of "Part:BBa K1631012:Experience"
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===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1631012 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1631012 StartReviews</partinfo> | ||
| − | We succeeded to control growth rate of <i>E | + | We succeeded to control growth rate of <i>E coli JM109</i> (which expresses LacI excessively). |
| − | We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. | + | <p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. |
| − | [[File:Yamanaka 002.png|600px|thumb| | + | [[File:Yamanaka 002.png|600px|thumb|center|alt text]] |
| − | + | </p> | |
We also evaluated the growth rate by slope of next graph. | We also evaluated the growth rate by slope of next graph. | ||
| − | [[File:Yamanaka 001.png|600px|thumb| | + | [[File:Yamanaka 001.png|600px|thumb|center|alt text]] |
| − | + | <br>Plac GFP- 0.0213(/min) | |
| − | + | <br>Plac GFP+ 0.0205(/min) | |
| − | Plac Barnase GFP- 0.0222 | + | <br>Plac Barnase GFP- 0.0222(/min) |
| − | Plac Barnase GFP+ 0.0117 | + | <br>Plac Barnase GFP+ 0.0117(/min) |
Latest revision as of 00:50, 19 September 2015
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UNIQf880e429ce8916c9-partinfo-00000000-QINU We succeeded to control growth rate of E coli JM109 (which expresses LacI excessively).
We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
We also evaluated the growth rate by slope of next graph.
Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)
UNIQf880e429ce8916c9-partinfo-00000001-QINU