Difference between revisions of "Part:BBa K632003:Experience"

Latest revision as of 00:41, 19 September 2015

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UNIQ135318ca3d78f5eb-partinfo-00000000-QINU

No review score entered. HZAU-China iGEM 2015

We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light. We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator.

Fig 1: The genetic circuit of test system.
Two sample owning the same genetic circuit were set in our test experiment, the one in dark, the other in red light, and each sample had three biologic repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture shown.
Fig 2:A red LED powered by battery is set at the bottom of a cup to provide red light

Fig 3:Two light conditions in our test device.

After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes. (In the test, we used GFP as the target gene).

Fig 4. The effect of red light on the expression of genes

More information, click here: http://2015.igem.org/Team:HZAU-China

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UNIQ135318ca3d78f5eb-partinfo-00000002-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K632003 StartReviews</partinfo>
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 We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light.
 We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator.
+https://static.igem.org/mediawiki/2015/0/06/Hzau1.jpg
+https://static.igem.org/mediawiki/2015/8/8a/Hzau2.jpg
 Fig 1: The genetic circuit of test system.
 <br>
 Two sample owning the same genetic circuit were set in our test experiment, the one in dark, the other in red light, and each sample had three biologic repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture shown.
+https://static.igem.org/mediawiki/2015/0/09/Hzau3.jpg
+<br>
 Fig 2:A red LED powered by battery is set at the bottom of a cup to provide red light
+https://static.igem.org/mediawiki/2015/f/fc/Hzau4.jpg
 Fig 3:Two light conditions in our test device.
 <br>
 After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes. (In the test, we used GFP as the target gene).
+https://static.igem.org/mediawiki/2015/2/29/Hzau5.jpg
 Fig 4. The effect of red light on the expression of genes
 <br>
-More information, click here: http://2015.igem.org/Team:HZAU-China/WetLab/Characterization
+More information, click here: http://2015.igem.org/Team:HZAU-China
 |};
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K632003 EndReviews</partinfo>