Difference between revisions of "Part:BBa K1688001"

Latest revision as of 21:54, 18 September 2015

Mono-rhamnolipid genes

The enzymes responsible for producing the mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The rhlA gene (Ba_K1688002) coding for RhlA enzyme creates a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the rhlB gene (BBa_K1688003) coding for the enzyme RhlB connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick (BBa_K1688001) is a composite part of BBa_K1688002 and BBa_K1688003.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 87
Illegal BamHI site found at 647
Illegal XhoI site found at 823
Illegal XhoI site found at 2137
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1030
Illegal NgoMIV site found at 1751
Illegal NgoMIV site found at 1864
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 312
Illegal BsaI site found at 1380
Illegal BsaI.rc site found at 496

Revision as of 21:50, 18 September 2015 (view source) Sangeethabupalan (Talk \| contribs) ← Older edit		Latest revision as of 21:54, 18 September 2015 (view source) Sangeethabupalan (Talk \| contribs)
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	<partinfo>BBa_K1688001 short</partinfo>		<partinfo>BBa_K1688001 short</partinfo>

−	The enzymes responsible for producing the mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The ~~RhlA~~ gene ([https://parts.igem.org/Part:BBa_K1688002 Ba_K1688002]) ~~codes~~ for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the ~~RhlB~~ ([https://parts.igem.org/Part:BBa_K1688003 BBa_K1688003]) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick ([https://parts.igem.org/Part:BBa_K1688001 BBa_K1688001]) is a composite part of [https://parts.igem.org/Part:BBa_K1688002 BBa_K1688002] and [https://parts.igem.org/Part:BBa_K1688003 BBa_K1688003].	+	The enzymes responsible for producing the mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The <i>rhlA</i> gene ([https://parts.igem.org/Part:BBa_K1688002 Ba_K1688002]) coding for RhlA enzyme creates a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the <i>rhlB</i> gene ([https://parts.igem.org/Part:BBa_K1688003 BBa_K1688003]) coding for the enzyme RhlB connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick ([https://parts.igem.org/Part:BBa_K1688001 BBa_K1688001]) is a composite part of [https://parts.igem.org/Part:BBa_K1688002 BBa_K1688002] and [https://parts.igem.org/Part:BBa_K1688003 BBa_K1688003].