Difference between revisions of "Part:BBa K1688003"

Latest revision as of 22:26, 18 September 2015

RhlB Gene

The enzymes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The rhlA gene (Ba_K1688002) coding for the enzyme RhlA creates the fatty acid tail and the enzyme RhlB from rhlB gene connects the tail to a rhamnose molecule. The rhlB gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomonas aeruginosa. The biobrick Ba_K1688003 is an assembly of RBS with BBa_K1331004 that codes for RhlB enzyme

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1164
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 57
Illegal NgoMIV site found at 778
Illegal NgoMIV site found at 891
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 407

Revision as of 21:42, 18 September 2015 (view source) Sangeethabupalan (Talk \| contribs) ← Older edit		Latest revision as of 22:26, 18 September 2015 (view source) Haer8191 (Talk \| contribs) m
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−	The enzymes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The <i>rhlA</i> gene ([https://parts.igem.org/Part:BBa_K1688002 Ba_K1688002]) coding for the enzyme RhlA creates the fatty acid tail and the enzyme RhlB from <i>rhlB</i> gene connects the tail to a rhamnose molecule. The <i>rhlB</i> gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from <i>''~~Pseudomona~~ aeruginosa''</i>. The biobrick ([https://parts.igem.org/Part:BBa_K1688003 Ba_K1688003]) is an assembly of RBS with ([https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004])that codes for RhlB enzyme	+	The enzymes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The <i>rhlA</i> gene ([https://parts.igem.org/Part:BBa_K1688002 Ba_K1688002]) coding for the enzyme RhlA creates the fatty acid tail and the enzyme RhlB from <i>rhlB</i> gene connects the tail to a rhamnose molecule. The <i>rhlB</i> gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from <i>''Pseudomonas aeruginosa''</i>. The biobrick [https://parts.igem.org/Part:BBa_K1688003 Ba_K1688003] is an assembly of RBS with [https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004] that codes for RhlB enzyme