Difference between revisions of "Part:BBa K1688003"
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<partinfo>BBa_K1688003 short</partinfo>
 
<partinfo>BBa_K1688003 short</partinfo>
  
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The enzymes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The mono- rhamnolipids consist of a  hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene (BBa_K1688002) codes for the fatty acid tail and the RhlB connects the tail to a rhamnose molecule. The RhlB gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from <i>''Pseudomona aeruginosa''</i>. The  biobrick BBa_K1688003  is an assembly of RBS with BBa_K1331004
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The enzymes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The mono- rhamnolipids consist of a  hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The <i>rhlA</i> gene ([https://parts.igem.org/Part:BBa_K1688002 Ba_K1688002]) coding for the enzyme RhlA creates the fatty acid tail and the enzyme RhlB from <i>rhlB</i> gene connects the tail to a rhamnose molecule. The <i>rhlB</i> gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from <i>''Pseudomonas aeruginosa''</i>. The  biobrick [https://parts.igem.org/Part:BBa_K1688003 Ba_K1688003] is an assembly of RBS with [https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004] that codes for RhlB enzyme
  
  

Latest revision as of 22:26, 18 September 2015

RhlB Gene

The enzymes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The rhlA gene (Ba_K1688002) coding for the enzyme RhlA creates the fatty acid tail and the enzyme RhlB from rhlB gene connects the tail to a rhamnose molecule. The rhlB gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomonas aeruginosa. The biobrick Ba_K1688003 is an assembly of RBS with BBa_K1331004 that codes for RhlB enzyme


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 57
    Illegal NgoMIV site found at 778
    Illegal NgoMIV site found at 891
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 407