Difference between revisions of "Part:BBa K1800003:Design"
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===Source=== | ===Source=== | ||
| − | + | Pre-optimized sequence from Futoshi et.al, 2007 | |
Synthesized by IDT | Synthesized by IDT | ||
===References=== | ===References=== | ||
| + | |||
| + | Taura, Futoshi, et al. "Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa." FEBS letters 581.16 (2007): 2929-2934. | ||
Latest revision as of 20:41, 18 September 2015
CBDA Synthase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1271
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 541
Illegal NgoMIV site found at 1120 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was codon optimized for the tobacco plant strain Nicotiana tabacum using the codon optimization tool from IDT. Added was RFC 10 biobrick prefix and suffix. EcoRI, XbaI, SpeI, PstI restriction sites were removed from the coding sequence for iGEM compatibility. Includes native start codon.
Source
Pre-optimized sequence from Futoshi et.al, 2007
Synthesized by IDT
References
Taura, Futoshi, et al. "Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa." FEBS letters 581.16 (2007): 2929-2934.