Difference between revisions of "Part:BBa K1820001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <p> | + | <p> Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression and translation beginning from the RBS in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.</p> |
| + | <p> This construct has yet to be cloned and expressed in ''L. lactis'', and validated for functionality.</p> | ||
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Latest revision as of 16:13, 26 September 2015
P335-Φ31 inducible middle promoter + RBS
A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 (BBa_K1820000), containing two tandem transcription sites, and a downstream ribosomal binding site (RBS).
Usage and Biology
Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression and translation beginning from the RBS in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.
This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 42
References
Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.