Difference between revisions of "Part:BBa K1820000"
(→‎Usage and Biology)
 
(5 intermediate revisions by 2 users not shown)
Line 5: Line 5:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
<p> Transcption form this promoter is induced after phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.</p>
+
<p> Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.</p>
 +
 
 +
<p> This construct has yet to be cloned and expressed in ''L. lactis'', and validated for functionality.</p>
  
 
<!-- -->
 
<!-- -->
Line 11: Line 13:
 
===Sequence and Features===
 
===Sequence and Features===
 
<span class='h3bb'></span>
 
<span class='h3bb'></span>
−
<partinfo>BBa_K1820014 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K1820000 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K1820014 parameters</partinfo>
+
<partinfo>BBa_K1820000 parameters</partinfo>
 
<!-- -->
 
<!-- -->
  
 
===References===
 
===References===
 
<p>Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.</p>
 
<p>Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.</p>
−
<p>Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.</p>
 

Latest revision as of 16:12, 26 September 2015

P335-Φ31 inducible middle promoter

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites.

Usage and Biology

Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.

This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


References

Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.