Difference between revisions of "Part:BBa K1651000"
 
(2 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1651000 short</partinfo>
 
<partinfo>BBa_K1651000 short</partinfo>
  
−
When the PAI1 spread into the amplifier, it will combine with a protein named lasR which is regulated by a constitutive promoter to /n form a complex. The PAI1-lasR complex will then activate the pLAS to produce a protein named rhlI.The rhlI will catalyze the production of PAI2, which will combine with another protein coming from the rhlIR circuit. And the rhlR is also regulated by a constitutive promoter like lasR. Now there comes to the PAI2-rhlR complex.
+
When the PAI1(3-oxo-C12-HSL) spread into the amplifier, it will combine with LasR which is regulated by a constitutive promoter. Then the LasR & PAI regulated promoter will be activated to start the production of RhlI. The RhlI will catalyze the production of PAI2(C4-HSL), which will combine with RhlR to activate the RhlR & PAI2 regulated promoter. Then the production of sfGFP is activated.
  
−
The PAI2-rhlR complex will directly activate the pRHL to enable the production of super - folder GFP.
+
The detection limit of this detector is very low(0.005 nmol/L, see more data in experience).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:40, 19 September 2015

PAI1 detector

When the PAI1(3-oxo-C12-HSL) spread into the amplifier, it will combine with LasR which is regulated by a constitutive promoter. Then the LasR & PAI regulated promoter will be activated to start the production of RhlI. The RhlI will catalyze the production of PAI2(C4-HSL), which will combine with RhlR to activate the RhlR & PAI2 regulated promoter. Then the production of sfGFP is activated.

The detection limit of this detector is very low(0.005 nmol/L, see more data in experience).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
    Illegal NheI site found at 2604
    Illegal NheI site found at 2627
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1773
    Illegal BamHI site found at 2901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3376
    Illegal BsaI.rc site found at 2513