Difference between revisions of "Part:BBa K1713013"
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The hybrid promoter is activated by Arabinose in the presence of Arac protein and suppressed by lacI protein in tha absence of IPTG. | The hybrid promoter is activated by Arabinose in the presence of Arac protein and suppressed by lacI protein in tha absence of IPTG. | ||
| + | <p>Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.</p> | ||
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Latest revision as of 13:08, 20 January 2016
Plac/ara-1+B0032+lacI(+LVA)
The hybrid promoter is activated by Arabinose in the presence of Arac protein and suppressed by lacI protein in tha absence of IPTG.
Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1276
Illegal BamHI site found at 28 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]