Difference between revisions of "Part:BBa K1714001:Experience"
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
−
<p>This part will be finally introduced upstream of BBa_K1714004 (P<sub>BAD</sub>-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.</p>
+
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 +
how you used this part and how it worked out.
  
−
<p>In this page, I described the result of the activity assay of ABF-2.</p>
 
  
 
===Applications of BBa_K1714001===
 
===Applications of BBa_K1714001===
−
<p>We transformed it into <i>E. coli</i> (in this case, we used DH5α) and compared the growth curve between samples induced or not.</p>
 
−
<p>Activity test for ABF-2 and thanatin</p>
 
−
<p>→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.</p>
 
−
  <ol>
 
−
  <li>Diluting the bacterial culture which is enough pre-cultured by 0.1 OD<sub>600</sub> (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol) </li>
 
−
  <li>Adding 2 µL of IPTG into one and DW into the other.</li>
 
−
  <li>Measuring OD<sub>600</sub> of these samples once in an hour. </li>
 
−
  </ol>
 
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 17:18, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Applications of BBa_K1714001

User Reviews

UNIQ71a5149d6705b27f-partinfo-00000000-QINU UNIQ71a5149d6705b27f-partinfo-00000001-QINU