Difference between revisions of "Part:BBa K1789017"
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This part is positive control in GFP series. Use to comparison with other device in GFP series in light emission. In this part, GFP is normally expressed. It proves that we have no problems with our experimental system. And the Plac, RBS30, terminator is all working well.  
 
This part is positive control in GFP series. Use to comparison with other device in GFP series in light emission. In this part, GFP is normally expressed. It proves that we have no problems with our experimental system. And the Plac, RBS30, terminator is all working well.  
  
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GFP is a shared protein in biology experiment. We utilize its luminous properties to visually display that scaffold could make two proteins close enough.
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GFP is a shared protein in biology experiment. We utilize its fluorescence properties to visually display that scaffold could make two proteins close enough.
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+
  
 
==Sequence and Features==
 
==Sequence and Features==
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===Colony PCR Test===
 
===Colony PCR Test===
  
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[[File:GFP P.jpg|400px|]]
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[[File:GFP P.jpg|700px|]]
  
 
Fig. 1 results of Colony PCR Test of GFP_P. We use pSB1C3-F and pSB1C3-R as the forward primer and reverse primer in this colony PCR system.
 
Fig. 1 results of Colony PCR Test of GFP_P. We use pSB1C3-F and pSB1C3-R as the forward primer and reverse primer in this colony PCR system.
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===Fuctional Test===
 
===Fuctional Test===
  
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The plasmid was transformed into E.coli[BL21], and was cultured under the 1mM of IPTG induction overnight. We use it to draw some pictures to test whether it can emit visible fluorescence.
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The plasmid was transformed into ''E.coli'' BL21(DE3), and was cultured under the 1mM of IPTG induction overnight. We use it to draw some pictures to test whether it can emit visible fluorescence.
  
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[[File:GFP_P_F.jpg|450px|]]  
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[[File:GFP_P_F.jpg|400px|]]  
  
 
Fig. 2 Picture drawn with bacteria transformed by GFP_P
 
Fig. 2 Picture drawn with bacteria transformed by GFP_P
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===References===
 
===References===

Latest revision as of 02:23, 19 September 2015

GFP_P

This device is a positive control of GFP with scaffold.


Usage and Biology

This part is positive control in GFP series. Use to comparison with other device in GFP series in light emission. In this part, GFP is normally expressed. It proves that we have no problems with our experimental system. And the Plac, RBS30, terminator is all working well.

GFP is a shared protein in biology experiment. We utilize its fluorescence properties to visually display that scaffold could make two proteins close enough.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 873


Experimental Validation

Sequencing

This part is sequenced as correct after construction.

Colony PCR Test

GFP P.jpg

Fig. 1 results of Colony PCR Test of GFP_P. We use pSB1C3-F and pSB1C3-R as the forward primer and reverse primer in this colony PCR system.

Fuctional Test

The plasmid was transformed into E.coli BL21(DE3), and was cultured under the 1mM of IPTG induction overnight. We use it to draw some pictures to test whether it can emit visible fluorescence.

GFP P F.jpg

Fig. 2 Picture drawn with bacteria transformed by GFP_P

References